Hotspot

psbB-psbT

LSC · 80942-81198 · 257 bp

Back to Bruguiera

Pi 0.0467

MarkerSeek score 67.10

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 20
pi: 0.0467
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAAAAACTTGGCGATCCA	TCCCCTACAACAGTTCGTCT	45.0	50.0	58.0	58.0	746-867 bp	1.0	86.4
psbB-psbT_p2	ACTCTTTGTCCGTCGTATGC	TCCCCTACAACAGTTCGTCT	50.0	50.0	58.0	58.0	1171-1292 bp	1.0	85.5
psbB-psbT_p3	GGGATGGAATAGCTGTTGGG	TCCCCTACAACAGTTCGTCT	55.0	50.0	58.0	58.0	1232-1353 bp	1.0	85.3
psbB-psbT_p4	GGGGATGGAATAGCTGTTGG	TCCCCTACAACAGTTCGTCT	55.0	50.0	58.0	58.0	1233-1354 bp	1.0	85.3
psbB-psbT_p5	GCTGGGCTAGTCGAAAATCA	TCCCCTACAACAGTTCGTCT	50.0	50.0	58.0	58.0	1362-1483 bp	1.0	84.8