Hotspot

ndhC-trnV

LSC · 55569-57077 · 1509 bp

Back to Bruguiera

Pi 0.0122

MarkerSeek score 64.40

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1509
variable_sites: 38
pi: 0.0122
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	GCCCTTTGTTAATCGGGGAT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.9	57.9	1546-1663 bp	1.0	84.8
ndhC-trnV_p2	GCCCTTTGTTAATCGGGGAT	CAGGTCTACGGTTCGAATCC	50.0	55.0	57.9	57.8	1555-1672 bp	1.0	83.7
ndhC-trnV_p3	GCCCTTTGTTAATCGGGGAT	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.9	58.3	1547-1664 bp	1.0	82.9
ndhC-trnV_p4	TCTCGGGCCCTTTGTTAATC	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.6	57.9	1552-1669 bp	1.0	82.0
ndhC-trnV_p5	GCCCTTTGTTAATCGGGGAT	GCAGGTCTACGGTTCGAATC	50.0	55.0	57.9	58.4	1556-1673 bp	1.0	81.8