Hotspot

trnT-GGU-psbD

LSC · 34946-36945 · 2000 bp

Back to Bruguiera

Pi 0.0158

MarkerSeek score 62.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 2000
variable_sites: 71
pi: 0.0158
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	2133-2211 bp	1.0	84.3
trnT-GGU-psbD_p2	GGCCCTTTTAACTCAGTGGT	GGCCAGACCAACCTACAAAA	50.0	50.0	57.7	58.0	2171-2249 bp	1.0	84.2
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.3	58.0	2135-2213 bp	1.0	84.1
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	TAGGCCAGACCAACCTACAA	50.0	50.0	57.7	58.0	2173-2251 bp	1.0	84.0
trnT-GGU-psbD_p5	CGCTATGGTAAGGCGTAAGT	GGCCAGACCAACCTACAAAA	50.0	50.0	57.5	58.0	2144-2222 bp	1.0	82.5