Hotspot

atpF-atpH

LSC · 13564-14269 · 706 bp

Back to Bruguiera

Pi 0.0262

MarkerSeek score 72.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 42
pi: 0.0262
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCGACCCAAAGAAACGAAAG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	58.0	58.2	737-845 bp	1.0	86.0
atpF-atpH_p2	GCGACCCAAAGAAACGAAAG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	58.0	57.6	739-847 bp	1.0	84.7
atpF-atpH_p3	AGCGACCCAAAGAAACGAAA	GCCTGGTTGTAGCATTAGCA	45.0	50.0	58.3	58.2	738-846 bp	1.0	83.7
atpF-atpH_p4	ATTAAGCCCGAAACTCCCAG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.6	58.2	767-875 bp	1.0	82.8
atpF-atpH_p5	AGCGACCCAAAGAAACGAAA	TGGCCTGGTTGTAGCATTAG	45.0	50.0	58.3	57.6	740-848 bp	1.0	82.3