Hotspot

atpA-atpF

LSC · 12225-12278 · 54 bp

Back to Bruguiera

Pi 0.037

MarkerSeek score 55.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 54
variable_sites: 6
pi: 0.037
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TCATCGGCTCGAATGCTTAC	CAATGAAAGAAATAACCGATTAG	50.0	30.4	58.1	52.0	100-102 bp	1.0	44.8
atpA-atpF_p2	CTCATCGGCTCGAATGCTTA	CAATGAAAGAAATAACCGATTAG	50.0	30.4	57.9	52.0	101-103 bp	1.0	44.7
atpA-atpF_p3	ACTTGAAGTACGGTACCGGT	CAATGAAAGAAATAACCGATTAG	50.0	30.4	58.4	52.0	181-183 bp	1.0	42.6
atpA-atpF_p4	CCGTCGCCTACTTGAAGTAC	CAATGAAAGAAATAACCGATTAG	55.0	30.4	58.1	52.0	190-192 bp	1.0	42.5
atpA-atpF_p5	GTCGCCTACTTGAAGTACGG	CAATGAAAGAAATAACCGATTAG	55.0	30.4	58.1	52.0	188-190 bp	1.0	42.5