Hotspot

trnR-UCU-atpA

LSC · 10210-10700 · 491 bp

Back to Bruguiera

Pi 0.062

MarkerSeek score 76.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 69
pi: 0.062
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.0	58.0	1375-1531 bp	1.0	88.3
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	GCGAGTTGCTCAAACAATCC	55.0	50.0	58.1	58.0	1378-1534 bp	1.0	88.0
trnR-UCU-atpA_p3	TTTGCCAGCTTCCCTAAAGG	GCGAGTTGCTCAAACAATCC	50.0	50.0	58.1	58.0	1476-1632 bp	1.0	87.7
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	CTTTCTAAACAAGCGCAGGC	50.0	50.0	58.0	58.0	1838-1994 bp	1.0	87.2
trnR-UCU-atpA_p5	CCTAGCCTTCCAAGCTAACG	CTTTCTAAACAAGCGCAGGC	55.0	50.0	58.1	58.0	1841-1997 bp	1.0	87.0