Hotspot

trnK-UUU-trnQ-UUG

LSC · 4798-6793 · 1996 bp

Back to Bruguiera

Pi 0.0265

MarkerSeek score 63.60

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1996
variable_sites: 118
pi: 0.0265
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1928-2141 bp	1.0	86.7
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1927-2140 bp	1.0	86.7
trnK-UUU-trnQ-UUG_p3	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1914-2127 bp	1.0	84.4
trnK-UUU-trnQ-UUG_p4	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.6	58.1	1939-2152 bp	1.0	83.5
trnK-UUU-trnQ-UUG_p5	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	1937-2150 bp	1.0	82.2