Hotspot

psbA

LSC · 504-1565 · 1062 bp

Back to Brugmansia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGAGCAATAGCACCCTCTTG	AAAACTCGTGTGCTTGGGAG	55.0	50.0	58.3	58.7	1198 bp	1.0	71.9
psbA_p2	GGAGCAATAGCACCCTCTTG	AGAAAACTCGTGTGCTTGGG	55.0	50.0	58.3	58.7	1200 bp	1.0	71.9
psbA_p3	GGAGCAATAGCACCCTCTTG	GAAAACTCGTGTGCTTGGGA	55.0	50.0	58.3	58.7	1199 bp	1.0	71.8
psbA_p4	GGAGCAATAGCACCCTCTTG	AAACTCGTGTGCTTGGGAG	55.0	52.6	58.3	58.0	1197 bp	1.0	69.3
psbA_p5	GAGCAATAGCACCCTCTTGA	AAAACTCGTGTGCTTGGGAG	50.0	50.0	57.3	58.7	1197 bp	1.0	68.9