Hotspot

atpI

LSC · 14985-15734 · 750 bp

Back to Brugmansia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 750
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TGAGCCACCCACCATATACA	TTTTCAGAGGGCAAGGCAAT	50.0	45.0	58.1	58.0	828 bp	1.0	79.0
atpI_p2	AATTCTCTTGAGCCACCCAC	TTTTCAGAGGGCAAGGCAAT	50.0	45.0	57.8	58.0	836 bp	1.0	78.2
atpI_p3	TGAGCCACCCACCATATACA	TTTTTCAGAGGGCAAGGCAA	50.0	45.0	58.1	58.2	829 bp	1.0	77.4
atpI_p4	TCTTGAGCCACCCACCATAT	TTTTCAGAGGGCAAGGCAAT	50.0	45.0	58.4	58.0	831 bp	1.0	76.7
atpI_p5	AATTCTCTTGAGCCACCCAC	TTTTTCAGAGGGCAAGGCAA	50.0	45.0	57.8	58.2	837 bp	1.0	76.6