Hotspot

atpF

LSC · 11938-13198 · 1261 bp

Back to Brugmansia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1261
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCGTCAGCTCGAATGGTT	GGGCGAAGTGCTACAAAAAG	45.0	50.0	57.8	57.7	1402 bp	1.0	76.0
atpF_p2	TCGTCAGCTCGAATGGTTAC	GGGCGAAGTGCTACAAAAAG	50.0	50.0	57.7	57.7	1400 bp	1.0	75.2
atpF_p3	TTTCGTCAGCTCGAATGGTT	TAAAAAGAGGGGCGAAGTGC	45.0	50.0	57.8	58.5	1411 bp	1.0	74.9
atpF_p4	GTCAGCTCGAATGGTTACCA	GGGCGAAGTGCTACAAAAAG	50.0	50.0	57.6	57.7	1398 bp	1.0	74.3
atpF_p5	ATTTCGTCAGCTCGAATGGT	GGGCGAAGTGCTACAAAAAG	45.0	50.0	57.6	57.7	1403 bp	1.0	74.3