Hotspot

atpA

LSC · 10360-11883 · 1524 bp

Back to Brugmansia

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	CGATAGCAAGAAAGACTGTTTGA	CCAATATTGGCATGTTGGGA	39.1	45.0	57.2	56.0	1712 bp	1.0	40.0
atpA_p2	TCGATAGCAAGAAAGACTGTTTG	CCAATATTGGCATGTTGGGA	39.1	45.0	57.2	56.0	1713 bp	1.0	40.0
atpA_p3	TCGATAGCAAGAAAGACTGT	CCAATATTGGCATGTTGGGA	40.0	45.0	54.1	56.0	1713 bp	1.0	40.0
atpA_p4	TCGATAGCAAGAAAGACTGTT	CCAATATTGGCATGTTGGGA	38.1	45.0	55.0	56.0	1713 bp	1.0	40.0
atpA_p5	TTCGATAGCAAGAAAGACTGT	CCAATATTGGCATGTTGGGA	38.1	45.0	55.0	56.0	1714 bp	1.0	40.0