Hotspot

ycf3

LSC · 44716-46745 · 2030 bp

Back to Brownlowia

Pi 0.000986
MarkerSeek score 52.30
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
2030
variable_sites
2
pi
0.000986
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TAAACAGAGTGCTCTTGTTC TGGAATGGGTCTGCTTTTATGT 40.0 40.9 53.2 58.0 2161-2162 bp 1.0 40.1
ycf3_p2 TAAACAGAGTGCTCTTGTTC CACGAGGGGTAATGAAAAGAGT 40.0 45.5 53.2 58.1 2091-2092 bp 1.0 40.1
ycf3_p3 TAAACAGAGTGCTCTTGTTC TCTCACGAGGGGTAATGAAA 40.0 45.0 53.2 55.9 2094-2095 bp 1.0 40.1
ycf3_p4 TAAACAGAGTGCTCTTGTTC TTCTCACGAGGGGTAATGAA 40.0 45.0 53.2 55.9 2095-2096 bp 1.0 40.1
ycf3_p5 TAAACAGAGTGCTCTTGTTC TTTCTCACGAGGGGTAATGA 40.0 45.0 53.2 55.9 2096-2097 bp 1.0 40.1