Hotspot

rpl16

LSC · 85237-86794 · 1558 bp

Back to Brownlowia

Pi 0.000642
MarkerSeek score 59.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1558
variable_sites
1
pi
0.000642
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GGAAACCCGCAATTGTTTTT TGAGCAGTTATCAATTCTATAAGGT 40.0 32.0 55.9 55.9 1677 bp 1.0 40.0
rpl16_p2 GGAAACCCGCAATTGTTTTT TGAGCAGTTATCAATTCTATAAGGTT 40.0 30.8 55.9 56.5 1677 bp 1.0 40.0
rpl16_p3 TCCCAAGACTTCTTTCCTTTGT TGAGCAGTTATCAATTCTATAAGGT 40.9 32.0 57.5 55.9 1652 bp 1.0 40.0
rpl16_p4 GGAAACCCGCAATTGTTTTT ATGAGCAGTTATCAATTCTATAAGGT 40.0 30.8 55.9 56.3 1678 bp 1.0 40.0
rpl16_p5 GGAAACCCGCAATTGTTTTT TGAGCAGTTATCAATTCTATAAGG 40.0 33.3 55.9 54.3 1677 bp 1.0 40.0