Hotspot

psbM

LSC · 30973-31077 · 105 bp

Back to Brownlowia

Pi 0.00952

MarkerSeek score 58.00

Primer available yes

Label psbM

Feature Metadata

label_name: psbM
feature_type: gene
region: LSC
length_bp: 105
variable_sites: 1
pi: 0.00952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM_p1	ACTGCTGATTCTAGTCCATTCA	ATCCCGAGTTATTGCGAAGT	40.9	45.0	56.9	57.0	236 bp	1.0	47.7
psbM_p2	GGATACTGCTGATTCTAGTCCA	ATCCCGAGTTATTGCGAAGT	45.5	45.0	56.8	57.0	240 bp	1.0	46.8
psbM_p3	AGACGTGAAATTGGAATCCT	ATCCCGAGTTATTGCGAAGT	40.0	45.0	54.7	57.0	205 bp	1.0	45.7
psbM_p4	ACTGCTGATTCTAGTCCATTCA	AATCCCGAGTTATTGCGAAGT	40.9	42.9	56.9	57.7	237 bp	1.0	45.4
psbM_p5	GGATACTGCTGATTCTAGTCCA	AATCCCGAGTTATTGCGAAGT	45.5	42.9	56.8	57.7	241 bp	1.0	44.5