Hotspot

psbB

LSC · 76397-77923 · 1527 bp

Back to Brownlowia

Pi 0.000655

MarkerSeek score 59.10

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 1
pi: 0.000655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	AAGAATCCACCGAAAAGGGG	TTCCAGCAGCCTATGTCAAA	50.0	45.0	57.8	57.1	1673 bp	1.0	71.3
psbB_p2	AAGAATCCACCGAAAAGGGG	TTTCCAGCAGCCTATGTCAA	50.0	45.0	57.8	57.1	1674 bp	1.0	71.3
psbB_p3	AAGAATCCACCGAAAAGGGG	TTTTCCAGCAGCCTATGTCA	50.0	45.0	57.8	57.1	1675 bp	1.0	71.3
psbB_p4	CCGAAAAGGGGAGGTATTCC	TTCCAGCAGCCTATGTCAAA	55.0	45.0	57.7	57.1	1664 bp	1.0	70.3
psbB_p5	CCGAAAAGGGGAGGTATTCC	TTTCCAGCAGCCTATGTCAA	55.0	45.0	57.7	57.1	1665 bp	1.0	70.3