Hotspot

rpl32-trnL-UAG

SSC · 117417-118538 · 1122 bp

Back to Brownlowia

Pi 0.000891

MarkerSeek score 59.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1122
variable_sites: 1
pi: 0.000891
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1237 bp	1.0	78.7
rpl32-trnL-UAG_p2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1238 bp	1.0	78.7
rpl32-trnL-UAG_p3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	1247 bp	1.0	77.7
rpl32-trnL-UAG_p4	AAGAAAGGATATTGGGCGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	1253 bp	1.0	77.5
rpl32-trnL-UAG_p5	AAGAAAGGATATTGGGCGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	1254 bp	1.0	77.5