Hotspot

ndhF-rpl32

SSC · 116231-117251 · 1021 bp

Back to Brownlowia

Pi 0

MarkerSeek score 58.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1021
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAGTGGCACAAAAGGTA	TGAAAAAGCTTTCAACGCCG	50.0	45.0	58.0	58.2	1170-1171 bp	1.0	78.7
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	TGAAAAAGCTTTCAACGCCG	45.0	45.0	58.0	58.2	1218-1219 bp	1.0	78.3
ndhF-rpl32_p3	GGGAAGTGGCACAAAAGGTA	GCCGCCCAATATCCTTTCTT	50.0	50.0	58.0	58.3	1154-1155 bp	1.0	77.5
ndhF-rpl32_p4	TGTTGCCGTCGGAAAAAGTA	GCCGCCCAATATCCTTTCTT	45.0	50.0	58.0	58.3	1202-1203 bp	1.0	77.2
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TGAAAAAGCTTTCAACGCCG	45.0	45.0	57.8	58.2	1220-1221 bp	1.0	77.2