Hotspot

petA-psbJ

LSC · 67023-68034 · 1012 bp

Back to Brownlowia

Pi 0

MarkerSeek score 60.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTACGTGTCCAAGGCCTTTT	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.1	57.6	1133-1152 bp	1.0	77.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.8	57.6	1121-1140 bp	1.0	76.7
petA-psbJ_p3	GATCCACTACGTGTCCAAGG	ATTCTGGATTGGGTTCGTCC	55.0	50.0	57.7	57.6	1139-1158 bp	1.0	75.6
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.4	57.6	1132-1151 bp	1.0	74.8
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	ATTCTGGATTGGGTTCGTCC	45.0	50.0	57.5	57.6	1128-1147 bp	1.0	74.4