Hotspot

trnM-CAU-atpE

LSC · 56091-56328 · 238 bp

Back to Brownlowia

Pi 0.0042

MarkerSeek score 54.90

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 1
pi: 0.0042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	AGAGGCAAACAATTGAGGCA	50.0	45.0	58.4	58.0	364 bp	1.0	76.9
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	CGAGTAGAGGCTATCAACGC	50.0	55.0	58.4	58.0	317 bp	1.0	76.6
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	GAGTAGAGGCTATCAACGCG	50.0	55.0	58.4	58.0	316 bp	1.0	76.6
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	CTCTTAGACGGGCTAGGACA	50.0	55.0	58.4	57.9	337 bp	1.0	76.5
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	ACTTGAGGAAAGCTGAAGGC	50.0	50.0	58.4	58.1	385 bp	1.0	76.1