Hotspot

ndhC-trnV-UAC

LSC · 54039-55163 · 1125 bp

Back to Brownlowia

Pi 0

MarkerSeek score 58.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1125
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1268-1269 bp	1.0	77.0
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1256-1257 bp	1.0	76.9
ndhC-trnV-UAC_p3	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1258-1259 bp	1.0	75.1
ndhC-trnV-UAC_p4	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1259-1260 bp	1.0	75.1
ndhC-trnV-UAC_p5	ACCCTTTCTAATCGGGGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.8	57.5	1267-1268 bp	1.0	74.2