Hotspot

ycf3-trnS-GGA

LSC · 46746-47704 · 959 bp

Back to Brownlowia

Pi 0.00104

MarkerSeek score 59.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 959
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	TGGCGAATTAGGTGAAGGTG	47.6	50.0	58.0	57.9	1088 bp	1.0	70.9
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTAGGTGAAGGTGCGGAAAG	47.6	50.0	58.0	57.8	1081 bp	1.0	70.3
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	1019 bp	1.0	70.2
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	GTGCGGAAAGAGAGGGATTC	47.6	55.0	58.0	58.3	1071 bp	1.0	69.0
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	GAATTAGGTGAAGGTGCGGA	47.6	50.0	58.0	57.6	1084 bp	1.0	68.6