Hotspot

rps14-psaB

LSC · 39216-39343 · 128 bp

Back to Brownlowia

Pi 0.0394
MarkerSeek score 75.90
Primer available yes
Label rps14-psaB

Feature Metadata

label_name
rps14-psaB
feature_type
igs
region
LSC
length_bp
128
variable_sites
5
pi
0.0394
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14-psaB_p1 TTCTCACTCAACGACGGAAC ACCAGTGGCTCTTTCCATTG 50.0 50.0 57.9 58.1 364-365 bp 1.0 79.6
rps14-psaB_p2 TTCTCACTCAACGACGGAAC CCAGTGGCTCTTTCCATTGT 50.0 50.0 57.9 58.1 363-364 bp 1.0 79.6
rps14-psaB_p3 CTCACTCAACGACGGAACTT ACCAGTGGCTCTTTCCATTG 50.0 50.0 57.9 58.1 362-363 bp 1.0 79.6
rps14-psaB_p4 CTCACTCAACGACGGAACTT CCAGTGGCTCTTTCCATTGT 50.0 50.0 57.9 58.1 361-362 bp 1.0 79.6
rps14-psaB_p5 TTCTCACTCAACGACGGAAC GCTCTTTCCATTGTGCAAGC 50.0 50.0 57.9 58.3 357-358 bp 1.0 78.0