Hotspot

petN-psbM

LSC · 29825-30972 · 1148 bp

Back to Brownlowia

Pi 0

MarkerSeek score 45.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGTACAAGGAAGGCGGTAGA	45.0	50.0	58.0	58.1	1637-1641 bp	1.0	79.6
petN-psbM_p2	TAGTGGAAGGGAAGCAGTCA	TGTACAAGGAAGGCGGTAGA	50.0	50.0	58.0	58.1	2059-2063 bp	1.0	79.4
petN-psbM_p3	AGTAGTGGAAGGGAAGCAGT	TGTACAAGGAAGGCGGTAGA	50.0	50.0	58.0	58.1	2061-2065 bp	1.0	79.4
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGATTGTACAAGGAAGGCGG	45.0	50.0	58.0	57.9	1641-1645 bp	1.0	79.3
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	GATTGTACAAGGAAGGCGGT	45.0	50.0	58.0	57.9	1640-1644 bp	1.0	79.3