Hotspot

atpF-atpH

LSC · 13413-14044 · 632 bp

Back to Brownlowia

Pi 0

MarkerSeek score 59.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	TGGTTGTAGCATTGGCACTT	50.0	45.0	58.0	58.0	715-717 bp	1.0	79.9
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGTTGTAGCATTGGCACTT	50.0	45.0	57.9	58.0	743-745 bp	1.0	79.2
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	CTGGTTGTAGCATTGGCACT	50.0	50.0	58.0	58.5	716-718 bp	1.0	76.2
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	ATGGGCTGGTTGTAGCATTG	50.0	50.0	58.0	58.5	721-723 bp	1.0	75.7
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CTGGTTGTAGCATTGGCACT	50.0	50.0	57.9	58.5	744-746 bp	1.0	75.6