Hotspot

rps16

LSC · 5266-6455 · 1190 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1190
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCCACACCCTCTTTTGTTTCT	TGGCTCCAAACCAGAGAAAT	42.9	45.0	57.3	57.0	1241 bp	1.0	58.9
rps16_p2	GGGTATGAATAGATAGCGGGG	TGGCTCCAAACCAGAGAAAT	52.4	45.0	57.2	57.0	1307 bp	1.0	57.6
rps16_p3	GGTATGAATAGATAGCGGGGG	TGGCTCCAAACCAGAGAAAT	52.4	45.0	57.2	57.0	1306 bp	1.0	57.6
rps16_p4	TCCACACCCTCTTTTGTTTCT	GGCTCCAAACCAGAGAAATG	42.9	50.0	57.3	56.7	1240 bp	1.0	56.2
rps16_p5	TCCACACCCTCTTTTGTTTCTT	TGGCTCCAAACCAGAGAAAT	40.9	45.0	58.0	57.0	1241 bp	1.0	56.0