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petD

LSC · 78936-80172 · 1237 bp

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Pi 0.000808

MarkerSeek score 59.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1237
variable_sites: 1
pi: 0.000808
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGTTTCCCCTCAACTTCACA	CGTTTCAAAGCGAATTCTCCC	45.0	47.6	56.9	58.1	1400 bp	1.0	62.0
petD_p2	GGATGTTTCCCCTCAACTTCA	CGTTTCAAAGCGAATTCTCCC	47.6	47.6	57.8	58.1	1403 bp	1.0	61.6
petD_p3	TGGATGTTTCCCCTCAACTTC	CGTTTCAAAGCGAATTCTCCC	47.6	47.6	57.8	58.1	1404 bp	1.0	61.6
petD_p4	TGGATGTTTCCCCTCAACTT	CGTTTCAAAGCGAATTCTCCC	45.0	47.6	56.6	58.1	1404 bp	1.0	59.9
petD_p5	TTGGATGTTTCCCCTCAACT	CGTTTCAAAGCGAATTCTCCC	45.0	47.6	56.6	58.1	1405 bp	1.0	59.9