Hotspot

petB

LSC · 77260-78731 · 1472 bp

Back to Brownea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1472
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GTAGTCTGACCGCGGAATTT	CTCCCCCAAACGAGAAATGA	50.0	50.0	58.0	57.5	1565 bp	1.0	76.0
petB_p2	GTAGTCTGACCGCGGAATTT	CCTCCCCCAAACGAGAAATG	50.0	55.0	58.0	58.5	1566 bp	1.0	75.7
petB_p3	GTAGTCTGACCGCGGAATTT	TCCTCCCCCAAACGAGAAAT	50.0	50.0	58.0	58.6	1567 bp	1.0	74.9
petB_p4	TCTGACCGCGGAATTTCTTT	CTCCCCCAAACGAGAAATGA	45.0	50.0	57.8	57.5	1561 bp	1.0	74.6
petB_p5	GTAGTCTGACCGCGGAATTT	ACTATCCTTCCTCCCCCAAA	50.0	50.0	58.0	57.3	1575 bp	1.0	74.5