Hotspot

matK

LSC · 2101-3618 · 1518 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACTTACTCCATGAACATTTCA	TCCCCTATCCCTGTCTCAAA	33.3	50.0	52.8	57.0	1671 bp	1.0	40.0
matK_p2	ACTTACTCCATGAACATTTCA	CCCTGTTTTGACTGTATCGC	33.3	50.0	52.8	56.8	1719 bp	1.0	40.0
matK_p3	ACTTACTCCATGAACATTTCA	AATCCCCTATCCCTGTCTCA	33.3	50.0	52.8	56.8	1673 bp	1.0	40.0
matK_p4	ACTTACTCCATGAACATTTCA	ATCCCCTATCCCTGTCTCAA	33.3	50.0	52.8	56.8	1672 bp	1.0	40.0
matK_p5	ACTTACTCCATGAACATTTCA	TCCCCTATCCCTGTCTCAAAT	33.3	47.6	52.8	57.6	1671 bp	1.0	40.0