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psbM-trnD-GUC

LSC · 31587-32504 · 918 bp

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Pi 0

MarkerSeek score 26.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 918
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	GTTCGAGCCCCGTCAGTC	45.0	66.7	55.8	60.1	986 bp	1.0	40.0
psbM-trnD-GUC_p2	AGGAACTAGAATGAACAGTGCA	GTTCGAGCCCCGTCAGTC	40.9	66.7	57.4	60.1	993 bp	1.0	40.0
psbM-trnD-GUC_p3	GCAGTAGCAATAAACGCTAGA	GTTCGAGCCCCGTCAGTC	42.9	66.7	56.2	60.1	974 bp	1.0	40.0
psbM-trnD-GUC_p4	GGAACTAGAATGAACAGTGCAG	GTTCGAGCCCCGTCAGTC	45.5	66.7	57.1	60.1	992 bp	1.0	40.0
psbM-trnD-GUC_p5	GGCAGTAGGAACTAGAATGAACA	GTTCGAGCCCCGTCAGTC	43.5	66.7	57.9	60.1	999 bp	1.0	40.0