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trnK-UUU-rps16

LSC · 4386-5265 · 880 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
880
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGCTGGTATGAACGAATCCA CAAAAAGGGCGGGTGTTTTT 50.0 45.0 58.1 58.0 1032 bp 1.0 79.2
trnK-UUU-rps16_p2 TCGCTGGTATGAACGAATCC CAAAAAGGGCGGGTGTTTTT 50.0 45.0 57.8 58.0 1033 bp 1.0 78.0
trnK-UUU-rps16_p3 CGCTGGTATGAACGAATCCA GGCGGGTGTTTTTATCGAAC 50.0 50.0 58.1 57.7 1025 bp 1.0 77.5
trnK-UUU-rps16_p4 TCGCTGGTATGAACGAATCC GGCGGGTGTTTTTATCGAAC 50.0 50.0 57.8 57.7 1026 bp 1.0 76.2
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG CAAAAAGGGCGGGTGTTTTT 50.0 45.0 58.6 58.0 984 bp 1.0 75.2