Hotspot

rpl32-trnL-UAG

SSC · 118549-120312 · 1764 bp

Back to Broussonetia

Pi 0.0376
MarkerSeek score 74.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1764
variable_sites
156
pi
0.0376
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGTAAATCTCTTTCTACAAGGA AGCGTGTCTACCGATTTCAC 34.8 50.0 54.5 58.0 1851-1983 bp 1.0 49.6
rpl32-trnL-UAG_p2 AGGTAAATCTCTTTCTACAAGGA CAGCGTGTCTACCGATTTCA 34.8 50.0 54.5 58.0 1852-1984 bp 1.0 49.6
rpl32-trnL-UAG_p3 AGGTAAATCTCTTTCTACAAGGA TCCTAAGAGCAGCGTGTCTA 34.8 50.0 54.5 57.9 1861-1993 bp 1.0 49.6
rpl32-trnL-UAG_p4 AGGTAAATCTCTTTCTACAAGGA CCTAAGAGCAGCGTGTCTAC 34.8 55.0 54.5 57.8 1860-1992 bp 1.0 49.6
rpl32-trnL-UAG_p5 AGGTAAATCTCTTTCTACAAGGA CTAAGAGCAGCGTGTCTACC 34.8 55.0 54.5 57.8 1859-1991 bp 1.0 49.6