Hotspot

petA-psbJ

LSC · 67579-68641 · 1063 bp

Back to Broussonetia

Pi 0.0192

MarkerSeek score 60.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1063
variable_sites: 50
pi: 0.0192
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	TTGTGATCGGGTTAATCGGT	42.9	45.0	57.2	56.9	1133-1164 bp	1.0	63.9
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGA	CTTGTGATCGGGTTAATCGGT	42.9	47.6	57.2	58.1	1134-1165 bp	1.0	63.6
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGA	TCTTGTGATCGGGTTAATCGG	42.9	47.6	57.2	57.8	1135-1166 bp	1.0	63.0
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGAA	TTGTGATCGGGTTAATCGGT	40.9	45.0	57.9	56.9	1133-1164 bp	1.0	61.3
petA-psbJ_p5	TTGAGAAGGTTCAATTGTCCGA	TTGTGATCGGGTTAATCGGT	40.9	45.0	57.9	56.9	1134-1165 bp	1.0	61.3