Hotspot

ycf4-cemA

LSC · 64840-65710 · 871 bp

Back to Broussonetia

Pi 0.0231

MarkerSeek score 55.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 871
variable_sites: 49
pi: 0.0231
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CAGAAAGCTGCTGAATTGGC	AACCCACGATTCAAGGCTTT	50.0	45.0	58.0	58.0	1036-1084 bp	0.285714	51.6
ycf4-cemA_p2	CAGAAAGCTGCTGAATTGGC	ACCCACGATTCAAGGCTTTT	50.0	45.0	58.0	58.0	1035-1083 bp	0.285714	51.6
ycf4-cemA_p3	AAATCGTCGCATCTTCCTCC	AACCCACGATTCAAGGCTTT	50.0	45.0	58.1	58.0	1202-1250 bp	0.285714	51.1
ycf4-cemA_p4	AAATCGTCGCATCTTCCTCC	ACCCACGATTCAAGGCTTTT	50.0	45.0	58.1	58.0	1201-1249 bp	0.285714	51.1
ycf4-cemA_p5	CATTCCCTTGACTCGTACCG	ACCCACGATTCAAGGCTTTT	55.0	45.0	58.1	58.0	1084-1132 bp	0.285714	51.0