Hotspot

trnT-UGU-trnL-UAA

LSC · 50129-51356 · 1228 bp

Back to Broussonetia

Pi 0.0251

MarkerSeek score 62.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 72
pi: 0.0251
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CGATGACCCTCGCATTACAA	GCGTCTACCTATTTCGCCAT	50.0	50.0	58.1	57.8	1279-1306 bp	1.0	85.3
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.9	57.8	1257-1284 bp	1.0	84.9
trnT-UGU-trnL-UAA_p3	AAAAGCCAGCTATCGGAGTC	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.7	57.8	1303-1330 bp	1.0	83.1
trnT-UGU-trnL-UAA_p4	GACCCTCGCATTACAAATGC	GCGTCTACCTATTTCGCCAT	50.0	50.0	57.5	57.8	1275-1302 bp	1.0	82.0
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	GCGTCTACCTATTTCGCCAT	55.0	50.0	57.5	57.8	1249-1276 bp	1.0	82.0