Hotspot

ycf3-trnS-GGA

LSC · 47757-48613 · 857 bp

Back to Broussonetia

Pi 0.0231
MarkerSeek score 64.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
857
variable_sites
46
pi
0.0231
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CTCTTTCTCGCCCGAAGTTG AGGTGAAGGGTACGGAAAGA 55.0 50.0 58.9 58.0 1058-1111 bp 1.0 77.4
ycf3-trnS-GGA_p2 CTCTTTCTCGCCCGAAGTTG CCCTCGGTAAACAAAAGCCT 55.0 50.0 58.9 58.1 1026-1079 bp 1.0 77.0
ycf3-trnS-GGA_p3 CTCTTTCTCGCCCGAAGTTG ACGGAAAGAGAGGGATTCGA 55.0 50.0 58.9 58.2 1047-1100 bp 1.0 76.4
ycf3-trnS-GGA_p4 CTTTCTCGCCCGAAGTTGT AGGTGAAGGGTACGGAAAGA 52.6 50.0 58.1 58.0 1056-1109 bp 1.0 76.2
ycf3-trnS-GGA_p5 TTTCTCGCCCGAAGTTGTC AGGTGAAGGGTACGGAAAGA 52.6 50.0 58.1 58.0 1055-1108 bp 1.0 76.1