Hotspot

psbC-trnS-UGA

LSC · 37946-38197 · 252 bp

Back to Broussonetia

Pi 0.03

MarkerSeek score 59.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 17
pi: 0.03
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	402-417 bp	1.0	80.0
psbC-trnS-UGA_p2	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	401-416 bp	1.0	79.5
psbC-trnS-UGA_p3	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	436-451 bp	1.0	79.4
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	413-428 bp	1.0	79.3
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.4	58.1	447-462 bp	1.0	78.7