Hotspot

atpF-atpH

LSC · 14069-14545 · 477 bp

Back to Broussonetia

Pi 0.0198

MarkerSeek score 62.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 24
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GATGGCCAATAACCCAAGGA	TGGACTCGTTGTGGCATTAG	50.0	50.0	57.5	57.9	563-581 bp	1.0	81.9
atpF-atpH_p2	GATGGCCAATAACCCAAGGA	TCGTTGTGGCATTAGCACTT	50.0	45.0	57.5	58.1	558-576 bp	1.0	81.8
atpF-atpH_p3	GGATGGCCAATAACCCAAGG	TGGACTCGTTGTGGCATTAG	55.0	50.0	58.6	57.9	564-582 bp	1.0	80.8
atpF-atpH_p4	GGATGGCCAATAACCCAAGG	TCGTTGTGGCATTAGCACTT	55.0	45.0	58.6	58.1	559-577 bp	1.0	80.7
atpF-atpH_p5	TGGCCAATAACCCAAGGAAA	TGGACTCGTTGTGGCATTAG	45.0	50.0	57.3	57.9	561-579 bp	1.0	79.9