Hotspot

atpA-atpF

LSC · 12705-12763 · 59 bp

Back to Broussonetia

Pi 0.0613

MarkerSeek score 62.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 8
pi: 0.0613
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TGGCATGTTTGGAACCATGA	50.0	45.0	58.0	58.0	212-214 bp	1.0	83.7
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	TGAAGTTCGACAACGGGTTT	50.0	45.0	58.0	58.0	317-319 bp	1.0	82.7
atpA-atpF_p3	ATGCCATCGCCTACTTGAAG	GCCCGCTTACGAAAAGTAGA	50.0	50.0	58.0	58.0	484-486 bp	1.0	82.5
atpA-atpF_p4	GTATTCCATTGCTCCCCGTT	GCCCGCTTACGAAAAGTAGA	50.0	50.0	57.9	58.0	1031-1033 bp	1.0	81.2
atpA-atpF_p5	GTATTCCATTGCTCCCCGTT	TGGCATGTTTGGAACCATGA	50.0	45.0	57.9	58.0	759-761 bp	1.0	81.1