Hotspot

rps16-trnQ-UUG

LSC · 6716-7671 · 956 bp

Back to Broussonetia

Pi 0.0264
MarkerSeek score 63.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
956
variable_sites
62
pi
0.0264
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACGTTGCCTTCTACCACATC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 874-1048 bp 1.0 87.5
rps16-trnQ-UUG_p2 GTTGCCTTCTACCACATCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 872-1046 bp 1.0 87.5
rps16-trnQ-UUG_p3 CACGTTGCCTTCTACCACAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 875-1049 bp 1.0 86.8
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 896-1070 bp 1.0 85.2
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 895-1069 bp 1.0 85.2