Hotspot

trnH-GUG-psbA

LSC · 76-456 · 381 bp

Back to Broussonetia

Pi 0.0449

MarkerSeek score 70.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 40
pi: 0.0449
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	AGAAGTTCCATCTACAAATGG	55.0	38.1	58.1	53.2	405-430 bp	1.0	51.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	AGAAGTTCCATCTACAAATGG	50.0	38.1	57.9	53.2	419-444 bp	1.0	51.4
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	AGAAGTTCCATCTACAAATGG	50.0	38.1	57.9	53.2	418-443 bp	1.0	51.4
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	AGAAGTTCCATCTACAAATGG	50.0	38.1	57.9	53.2	420-445 bp	1.0	51.4
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	AGAAGTTCCATCTACAAATGG	50.0	38.1	57.5	53.2	434-459 bp	1.0	51.2