Hotspot

ndhF

SSC · 103015-105234 · 2220 bp

Pi 0.00811

MarkerSeek score 48.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 35
pi: 0.00811
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	ATGGTACGGAAGCCTATCCC	45.0	55.0	58.0	58.7	2820-3000 bp	0.8	73.4
ndhF_p2	TCGCGGAAAACCGAACTATT	CGGAAGCCTATCCCCCATAT	45.0	55.0	57.9	58.4	2810-2999 bp	0.6	65.6
ndhF_p3	ATCGCGGAAAACCGAACTAT	CGGAAGCCTATCCCCCATAT	45.0	55.0	57.7	58.4	2811-3000 bp	0.6	63.9
ndhF_p4	AAAATCGCGGAAAACCGAAC	CGGAAGCCTATCCCCCATAT	45.0	55.0	58.0	58.4	2814-2994 bp	0.4	57.9
ndhF_p5	AATCGCGGAAAACCGAACTA	CGGAAGCCTATCCCCCATAT	45.0	55.0	57.9	58.4	2812-2992 bp	0.4	57.6