Hotspot

rpl32-trnL-UAG

SSC · 106289-107028 · 740 bp

Back to Bromus

Pi 0.0172
MarkerSeek score 72.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
740
variable_sites
23
pi
0.0172
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 719-845 bp 1.0 85.3
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 718-844 bp 1.0 85.3
rpl32-trnL-UAG_p3 AAGGGTTTTTCTGGGCAACA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 722-848 bp 1.0 84.8
rpl32-trnL-UAG_p4 AGGGTTTTTCTGGGCAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 58.1 58.1 721-847 bp 1.0 84.8
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 691-817 bp 1.0 84.4