Hotspot

ndhF-rpl32

SSC · 105235-106108 · 874 bp

Back to Bromus

Pi 0.0223
MarkerSeek score 77.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
874
variable_sites
37
pi
0.0223
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.91
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 894-967 bp 1.0 47.2
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 895-968 bp 1.0 47.2
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 896-969 bp 1.0 47.2
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 897-970 bp 1.0 47.2
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 921-994 bp 1.0 47.1