Hotspot

ndhH-ndhF

SSC · 103000-103014 · 15 bp

Back to Bromus

Pi 0.0867
MarkerSeek score 65.50
Primer available yes
Label ndhH-ndhF

Feature Metadata

label_name
ndhH-ndhF
feature_type
igs
region
SSC
length_bp
15
variable_sites
3
pi
0.0867
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-ndhF_p1 AAAATCGCGGAAAACCGAAC ATGGAGTGGGTCTTGCTAGT 45.0 50.0 58.0 58.0 213-323 bp 1.0 91.3
ndhH-ndhF_p2 AACACCCATCAATGCATGGT ATGGAGTGGGTCTTGCTAGT 45.0 50.0 58.1 58.0 317-427 bp 1.0 91.2
ndhH-ndhF_p3 ACCCATCAATGCATGGTGTT ATGGAGTGGGTCTTGCTAGT 45.0 50.0 58.1 58.0 314-424 bp 1.0 91.2
ndhH-ndhF_p4 TCGCGGAAAACCGAACTATT ATGGAGTGGGTCTTGCTAGT 45.0 50.0 57.9 58.0 209-319 bp 1.0 91.0
ndhH-ndhF_p5 CTTTTGGGAAAACGTCGACG ATGGAGTGGGTCTTGCTAGT 50.0 50.0 58.0 58.0 631-742 bp 1.0 89.5