Hotspot

rpl22-rps19

LSC · 81232-81310 · 79 bp

Back to Bromus

Pi 0.0329
MarkerSeek score 71.90
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
79
variable_sites
6
pi
0.0329
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CGAGTTTCCTTAGCCCACTT CAAATTGGGGGAATTCGTGC 50.0 50.0 57.5 58.0 225-228 bp 1.0 79.3
rpl22-rps19_p2 CGAGTTTCCTTAGCCCACTT GCAAATTGGGGGAATTCGTG 50.0 50.0 57.5 58.0 226-229 bp 1.0 79.3
rpl22-rps19_p3 CGAGTTTCCTTAGCCCACTT ATGGTAGGTCGCAAATTGGG 50.0 50.0 57.5 58.2 236-239 bp 1.0 77.3
rpl22-rps19_p4 CGAGTTTCCTTAGCCCACTT GTCGCAAATTGGGGGAATTC 50.0 50.0 57.5 57.7 229-232 bp 1.0 77.0
rpl22-rps19_p5 CTTGCGAGTTTCCTTAGCCC CAAATTGGGGGAATTCGTGC 55.0 50.0 58.9 58.0 229-232 bp 1.0 75.7