Hotspot

trnP-UGG-psaJ

LSC · 64874-65271 · 398 bp

Back to Bromus

Pi 0.0101
MarkerSeek score 64.00
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
398
variable_sites
9
pi
0.0101
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG ACGGGTGCTACAGAGAGATA 45.0 50.0 57.9 57.3 452-471 bp 1.0 78.1
trnP-UGG-psaJ_p2 CAAAACAAACGCGCTACCAA ACGGGTGCTACAGAGAGATA 45.0 50.0 58.2 57.3 453-472 bp 1.0 77.6
trnP-UGG-psaJ_p3 ATCTCGTCGCTACTGCCTAT ACGGGTGCTACAGAGAGATA 50.0 50.0 58.1 57.3 532-551 bp 1.0 77.5
trnP-UGG-psaJ_p4 TTGTACCCAAAACAAACGCG ACGGGTGCTACAGAGAGATA 45.0 50.0 57.8 57.3 460-479 bp 1.0 77.4
trnP-UGG-psaJ_p5 ATTGATCTCGTCGCTACTGC ACGGGTGCTACAGAGAGATA 50.0 50.0 57.9 57.3 536-555 bp 1.0 77.2