Hotspot

trnY-GUA-trnD-GUC

LSC · 15555-15903 · 349 bp

Back to Bromus

Pi 0.0252
MarkerSeek score 54.70
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
349
variable_sites
15
pi
0.0252
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC CGGTGCTCTGACCAATTGAA 50.0 50.0 58.0 58.5 472-483 bp 1.0 78.4
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 439-450 bp 1.0 76.1
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.6 58.5 438-449 bp 1.0 75.9
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGTGCTCTGACCAATTGAAC 50.0 50.0 58.0 57.0 471-482 bp 1.0 74.2
trnY-GUA-trnD-GUC_p5 GATGCCCGAGTGGTTAATGG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.7 58.5 457-468 bp 1.0 73.3