primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	GCTCAACCGACAAGAACTGT	20	20	50.0	50.0	58.6	58.4	6.4	0.0	0.987484	3912	4492	686-705	1.000000	76.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGTCGCACTTAAAAGCCGAG	GTGCTCAACCGACAAGAACT	20	20	50.0	50.0	58.6	58.4	6.4	0.0	0.987484	3912	4492	688-707	1.000000	76.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCGCACTTAAAAGCCGAG	TGCTCAACCGACAAGAACTG	20	20	50.0	50.0	58.6	58.4	6.4	0.0	0.988604	3912	4492	687-706	1.000000	76.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCGCACTTAAAAGCCGAGTA	GCTCAACCGACAAGAACTGT	20	20	45.0	50.0	57.3	58.4	3.0	0.0	1.145636	3912	4492	684-703	1.000000	75.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCGCACTTAAAAGCCGAGTA	GTGCTCAACCGACAAGAACT	20	20	45.0	50.0	57.3	58.4	3.0	0.0	1.145636	3912	4492	686-705	1.000000	75.4
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5577	6319	816-846	1.000000	80.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5577	6319	827-857	1.000000	80.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5577	6319	820-850	1.000000	79.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5577	6319	831-861	1.000000	79.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5577	6319	822-852	1.000000	77.8
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	20	20	45.0	55.0	58.0	58.1	0.0	5.0	0.148662	7647	8557	1051-1077	1.000000	82.2
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	7647	8557	1029-1055	1.000000	81.2
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	20	20	45.0	55.0	58.0	57.7	0.0	0.0	0.349331	7647	8557	1013-1039	1.000000	80.7
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	20	20	55.0	55.0	57.8	58.1	0.0	5.0	0.343212	7647	8557	1102-1128	1.000000	80.5
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	20	20	45.0	50.0	58.0	58.3	0.0	5.0	0.369381	7647	8557	1044-1070	1.000000	80.5
trnG-UCC-trnT-GGU_p1	trnG-UCC-trnT-GGU	1	ACGAATCACACTTTTACCACT	ACCACTGAGTTAAAAGGGCT	21	20	38.1	45.0	55.5	56.7	0.0	0.0	4.884212	13520	14739	1217-1300	1.000000	46.7
trnG-UCC-trnT-GGU_p2	trnG-UCC-trnT-GGU	2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	21	20	38.1	45.0	55.5	56.6	0.0	0.0	4.907539	13520	14739	1254-1337	1.000000	46.5
trnG-UCC-trnT-GGU_p3	trnG-UCC-trnT-GGU	3	GAACGAATCACACTTTTACCACT	ACCACTGAGTTAAAAGGGCT	23	20	39.1	45.0	57.3	56.7	0.0	0.0	5.016928	13520	14739	1219-1302	1.000000	45.8
trnG-UCC-trnT-GGU_p4	trnG-UCC-trnT-GGU	4	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	21	21	38.1	47.6	55.5	57.5	0.0	0.0	5.004851	13520	14739	1250-1333	1.000000	45.7
trnG-UCC-trnT-GGU_p5	trnG-UCC-trnT-GGU	5	ACGAATCACACTTTTACCACT	ACCGATGACTTATGCCTTACC	21	21	38.1	47.6	55.5	57.5	0.0	0.0	5.006487	13520	14739	1251-1334	1.000000	45.7
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	58.0	58.5	0.0	7.1	0.521454	15555	15903	472-483	1.000000	78.4
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	15555	15903	439-450	1.000000	76.1
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	15555	15903	438-449	1.000000	75.9
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	20	20	50.0	50.0	58.0	57.0	0.0	7.1	1.041608	15555	15903	471-482	1.000000	74.2
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	GATGCCCGAGTGGTTAATGG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.7	58.5	0.0	7.1	1.168334	15555	15903	457-468	1.000000	73.3
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	17667	18597	913-1044	1.000000	76.8
petN-trnC-GCA_p2	petN-trnC-GCA	2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	17667	18597	912-1043	1.000000	76.8
petN-trnC-GCA_p3	petN-trnC-GCA	3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	17667	18597	854-985	1.000000	76.7
petN-trnC-GCA_p4	petN-trnC-GCA	4	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	17667	18597	914-1045	1.000000	76.4
petN-trnC-GCA_p5	petN-trnC-GCA	5	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	17667	18597	915-1046	1.000000	76.3
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	20	20	50.0	50.0	58.1	58.0	14.6	0.0	0.109519	18669	19833	1222-1235	1.000000	82.7
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.250044	18669	19833	1229-1242	1.000000	81.6
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18669	19833	1237-1250	1.000000	80.4
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18669	19833	1236-1249	1.000000	80.4
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	20	20	50.0	45.0	58.1	57.6	14.6	13.0	0.442790	18669	19833	1301-1314	1.000000	79.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	22	20	36.4	55.0	56.1	57.9	0.0	0.0	3.962644	50259	51177	964-1074	1.000000	53.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCGTGAAGCAGAAACA	CGGTTCGAATCCGTATAGCC	22	20	36.4	55.0	56.1	58.3	0.0	22.8	4.192025	50259	51177	965-1075	1.000000	51.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CATATTCGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	21	20	38.1	55.0	54.6	57.9	0.0	0.0	4.464233	50259	51177	963-1073	1.000000	49.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ATCATATTCGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	23	20	34.8	55.0	56.6	57.9	0.0	0.0	4.466592	50259	51177	965-1075	1.000000	49.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCATATTCGTGAAGCAGAAACAT	GGTTCGAATCCGTATAGCCC	23	20	34.8	55.0	56.6	57.9	0.0	0.0	4.466592	50259	51177	964-1074	1.000000	49.0
rbcL-psaI_p1	rbcL-psaI	1	GCCGGTAGATACTATCGATT	GTCATTGCAATTGCCGGAAA	20	20	45.0	45.0	54.0	57.9	1.7	26.9	4.110798	56357	58069	869-1806	0.800000	45.5
rbcL-psaI_p2	rbcL-psaI	2	GCCGGTAGATACTATCGATT	GAAGTCATTGCAATTGCCGG	20	20	45.0	50.0	54.0	58.4	1.7	26.9	4.400963	56357	58069	872-1809	0.800000	43.2
rbcL-psaI_p3	rbcL-psaI	3	GCCGGTAGATACTATCGATT	AGAAGTCATTGCAATTGCCG	20	20	45.0	45.0	54.0	57.4	1.7	26.9	4.675524	56357	58069	873-1810	0.800000	41.0
rbcL-psaI_p4	rbcL-psaI	4	GCCGGTAGATACTATCGATT	AAGTCATTGCAATTGCCGGA	20	20	45.0	45.0	54.0	58.7	1.7	26.9	4.777503	56357	58069	871-1808	0.800000	40.2
rbcL-psaI_p5	rbcL-psaI	5	GCCGGTAGATACTATCGATT	AGTCATTGCAATTGCCGGAA	20	20	45.0	45.0	54.0	58.7	1.7	26.9	4.777503	56357	58069	870-1807	0.800000	40.2
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	ACGGGTGCTACAGAGAGATA	20	20	45.0	50.0	57.9	57.3	5.7	0.0	0.844845	64874	65271	452-471	1.000000	78.1
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CAAAACAAACGCGCTACCAA	ACGGGTGCTACAGAGAGATA	20	20	45.0	50.0	58.2	57.3	5.7	0.0	0.902103	64874	65271	453-472	1.000000	77.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	ATCTCGTCGCTACTGCCTAT	ACGGGTGCTACAGAGAGATA	20	20	50.0	50.0	58.1	57.3	0.0	0.0	0.832033	64874	65271	532-551	1.000000	77.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	TTGTACCCAAAACAAACGCG	ACGGGTGCTACAGAGAGATA	20	20	45.0	50.0	57.8	57.3	3.5	0.0	0.921259	64874	65271	460-479	1.000000	77.4
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	ATTGATCTCGTCGCTACTGC	ACGGGTGCTACAGAGAGATA	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.857848	64874	65271	536-555	1.000000	77.2
rpl22-rps19_p1	rpl22-rps19	1	CGAGTTTCCTTAGCCCACTT	CAAATTGGGGGAATTCGTGC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.483162	81232	81310	225-228	1.000000	79.3
rpl22-rps19_p2	rpl22-rps19	2	CGAGTTTCCTTAGCCCACTT	GCAAATTGGGGGAATTCGTG	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.483162	81232	81310	226-229	1.000000	79.3
rpl22-rps19_p3	rpl22-rps19	3	CGAGTTTCCTTAGCCCACTT	ATGGTAGGTCGCAAATTGGG	20	20	50.0	50.0	57.5	58.2	0.0	3.4	0.711702	81232	81310	236-239	1.000000	77.3
rpl22-rps19_p4	rpl22-rps19	4	CGAGTTTCCTTAGCCCACTT	GTCGCAAATTGGGGGAATTC	20	20	50.0	50.0	57.5	57.7	0.0	10.2	0.768087	81232	81310	229-232	1.000000	77.0
rpl22-rps19_p5	rpl22-rps19	5	CTTGCGAGTTTCCTTAGCCC	CAAATTGGGGGAATTCGTGC	20	20	55.0	50.0	58.9	58.0	0.0	0.0	0.921251	81232	81310	229-232	1.000000	75.7
ndhH_p1	ndhH	1	TTTGATGAGCCTCGCTTTGA	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.225190	102664	102999	443-553	1.000000	89.9
ndhH_p2	ndhH	2	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	20	20	50.0	50.0	58.0	58.0	34.6	0.0	0.054997	102664	102999	631-742	1.000000	89.5
ndhH_p3	ndhH	3	TCGACGTTTGCTGGCTTATT	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.238378	102664	102999	617-728	1.000000	88.2
ndhH_p4	ndhH	4	CTTTTGGGAAAACGTCGACG	TGGAGTGGGTCTTGCTAGTT	20	20	50.0	50.0	58.0	58.3	34.6	0.0	0.283699	102664	102999	630-741	1.000000	87.7
ndhH_p5	ndhH	5	GCCGGAAGATGGATGCATAT	ATGGAGTGGGTCTTGCTAGT	20	20	50.0	50.0	57.9	58.0	5.0	0.0	0.161513	102664	102999	1114-1225	1.000000	86.6
ndhH-ndhF_p1	ndhH-ndhF	1	AAAATCGCGGAAAACCGAAC	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	58.0	58.0	6.1	0.0	0.082426	103000	103014	213-323	1.000000	91.3
ndhH-ndhF_p2	ndhH-ndhF	2	AACACCCATCAATGCATGGT	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.100335	103000	103014	317-427	1.000000	91.2
ndhH-ndhF_p3	ndhH-ndhF	3	ACCCATCAATGCATGGTGTT	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.100335	103000	103014	314-424	1.000000	91.2
ndhH-ndhF_p4	ndhH-ndhF	4	TCGCGGAAAACCGAACTATT	ATGGAGTGGGTCTTGCTAGT	20	20	45.0	50.0	57.9	58.0	6.1	0.0	0.124878	103000	103014	209-319	1.000000	91.0
ndhH-ndhF_p5	ndhH-ndhF	5	CTTTTGGGAAAACGTCGACG	ATGGAGTGGGTCTTGCTAGT	20	20	50.0	50.0	58.0	58.0	34.6	0.0	0.054997	103000	103014	631-742	1.000000	89.5
ndhF_p1	ndhF	1	AAAATCGCGGAAAACCGAAC	ATGGTACGGAAGCCTATCCC	20	20	45.0	55.0	58.0	58.7	6.1	0.0	0.691648	103015	105234	2820-3000	0.800000	73.4
ndhF_p2	ndhF	2	TCGCGGAAAACCGAACTATT	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	57.9	58.4	6.1	0.0	0.498442	103015	105234	2810-2999	0.600000	65.6
ndhF_p3	ndhF	3	ATCGCGGAAAACCGAACTAT	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	57.7	58.4	6.1	0.0	0.720027	103015	105234	2811-3000	0.600000	63.9
ndhF_p4	ndhF	4	AAAATCGCGGAAAACCGAAC	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	58.0	58.4	6.1	0.0	0.455990	103015	105234	2814-2994	0.400000	57.9
ndhF_p5	ndhF	5	AATCGCGGAAAACCGAACTA	CGGAAGCCTATCCCCCATAT	20	20	45.0	55.0	57.9	58.4	6.1	0.0	0.498442	103015	105234	2812-2992	0.400000	57.6
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105235	106108	894-967	1.000000	47.2
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105235	106108	895-968	1.000000	47.2
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105235	106108	896-969	1.000000	47.2
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105235	106108	897-970	1.000000	47.2
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	20	24	55.0	29.2	58.1	55.3	0.0	0.0	6.833076	105235	106108	921-994	1.000000	47.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	106289	107028	719-845	1.000000	85.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	106289	107028	718-844	1.000000	85.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGTTTTTCTGGGCAACA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.185902	106289	107028	722-848	1.000000	84.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAACAA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.185902	106289	107028	721-847	1.000000	84.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	106289	107028	691-817	1.000000	84.4