Hotspot

psaC-ndhE

SSC · 122286-123086 · 801 bp

Back to Brocchinia

Pi 0.0561

MarkerSeek score 72.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 801
variable_sites: 81
pi: 0.0561
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CATGCTGCGAGTTGTTTCAG	GGGTCACTTCGATGGTTTGT	50.0	50.0	58.0	58.1	1387-1941 bp	1.0	87.4
psaC-ndhE_p2	TTCCTAGTCTCCAGCGGAAA	GGGTCACTTCGATGGTTTGT	50.0	50.0	58.1	58.1	1770-2329 bp	1.0	86.8
psaC-ndhE_p3	ACAACCGCGAATTTCGAGTA	GGGTCACTTCGATGGTTTGT	45.0	50.0	57.9	58.1	1866-2425 bp	1.0	86.7
psaC-ndhE_p4	CATGCTGCGAGTTGTTTCAG	ACTATGGCTCGGCAATAAGC	50.0	50.0	58.0	58.1	1166-1665 bp	0.833333	79.6
psaC-ndhE_p5	TTCCTAGTCTCCAGCGGAAA	ACTATGGCTCGGCAATAAGC	50.0	50.0	58.1	58.1	1549-2050 bp	0.833333	79.0