Hotspot

rpl36-infA

LSC · 83536-83640 · 105 bp

Back to Brocchinia

Pi 0.0419

MarkerSeek score 62.70

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 10
pi: 0.0419
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CTACGTCCATTCTTGCGTGA	ACTCTGCGACATCGAAAGAC	50.0	50.0	58.0	58.0	999-1005 bp	1.0	83.2
rpl36-infA_p2	CAAGAAACTACCCGACCTCG	ACTCTGCGACATCGAAAGAC	55.0	50.0	58.0	58.0	1086-1092 bp	1.0	83.0
rpl36-infA_p3	CAAGAAACTACCCGACCTCG	TTGGTTTCAACTCTGCGACA	55.0	45.0	58.0	58.0	1095-1101 bp	1.0	82.8
rpl36-infA_p4	TCTACGTCCATTCTTGCGTG	ACTCTGCGACATCGAAAGAC	50.0	50.0	58.0	58.0	1000-1006 bp	0.5	61.5
rpl36-infA_p5	CCCTGATCCACAACTGTACG	ACTCTGCGACATCGAAAGAC	55.0	50.0	58.0	58.0	1188-1191 bp	0.333333	53.9